HEK293 Cells

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Organism Homo sapiens

Suspension HEK293F cells (Invitrogen) grown in serum-free 293 Freestyle medium (Invitrogen).

Proteins

Gene Description Species Peptides
P10909 CLU Clusterin Homo sapiens 2 / 28
P01861 IGHG4 Immunoglobulin heavy constant gamma 4 Homo sapiens 8 / 24
P02790 HPX Hemopexin Homo sapiens 1 / 19
P16870 CPE Carboxypeptidase E Homo sapiens 14 / 14
Q08380 LGALS3BP Galectin-3-binding protein Homo sapiens 9 / 9
P13473 LAMP2 Lysosome-associated membrane glycoprotein 2 Homo sapiens 9 / 9
O75882 ATRN Attractin Homo sapiens 1 / 9
Q15293 RCN1 Reticulocalbin-1 Homo sapiens 7 / 7
Q9H3G5 CPVL Probable serine carboxypeptidase CPVL Homo sapiens 6 / 6
P11279 LAMP1 Lysosome-associated membrane glycoprotein 1 Homo sapiens 6 / 6
P36955 SERPINF1 Pigment epithelium-derived factor Homo sapiens 2 / 5
Q92542 NCSTN Nicastrin Homo sapiens 5 / 5
P01033 TIMP1 Metalloproteinase inhibitor 1 Homo sapiens 5 / 5
P14625 HSP90B1 Endoplasmin Homo sapiens 5 / 5
Q9UHG3 PCYOX1 Prenylcysteine oxidase 1 Homo sapiens 4 / 4
Q92820 GGH Gamma-glutamyl hydrolase Homo sapiens 4 / 4
Q9Y4L1 HYOU1 Hypoxia up-regulated protein 1 Homo sapiens 4 / 4
Q96AY3 FKBP10 Peptidyl-prolyl cis-trans isomerase FKBP10 Homo sapiens 3 / 3
Q92626 PXDN Peroxidasin homolog Homo sapiens 3 / 3
Q7Z4H8 POGLUT3 Protein O-glucosyltransferase 3 Homo sapiens 2 / 2

... further results

Glycans

Name Mol. Wt. Classification Peptides
G00912UN H5N4S2 2,222.783 N-Glycan 3 / 227
G59626AS H5N4S 1,931.688 N-Glycan 2 / 126
G88374WZ H5N4FS2 2,368.841 N-Glycan 4 / 82
G08293MJ H5N4FS 2,077.745 N-Glycan 4 / 71
G83555HU H5N4F 1,786.65 N-Glycan 2 / 40
G25987BV H3N4F 1,462.544 N-Glycan 2 / 33
G02815KT H5N2 1,234.433 N-Glycan 15 / 30
G59937CP H4N4F 1,624.597 N-Glycan 2 / 30
G41247ZX H6N2 1,396.486 N-Glycan 20 / 30
G85144OK H6N5FS2 2,733.973 N-Glycan 2 / 28
G62765YT H8N2 1,720.592 N-Glycan 17 / 22
G68318VE H4N5F 1,827.677 N-Glycan 2 / 21
G88725PI H3N5F 1,665.624 N-Glycan 2 / 19
G80920RR H9N2 1,882.645 N-Glycan 16 / 19
G40926MX H6N5S 2,296.82 N-Glycan 1 / 19
G12580WI H5N5FS 2,280.825 N-Glycan 3 / 19
G80475RE H3N3F 1,259.465 N-Glycan 2 / 16
G45504EY H4N4S 1,769.635 N-Glycan 1 / 14
G95678HJ H7N6FS4 3,681.296 N-Glycan 3 / 14
G44953PJ H4N5FS 2,118.772 N-Glycan 2 / 13

... further results

Acquisitions

Spectrum FileAnal. FractionMethodTrans. Groups
MS_47_UO1_HEK_1_1nSAX-ERLICH EnrichmentOrbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS4
MS_47_UO1_HEK_1_2nSAX-ERLICH EnrichmentOrbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS9
MS_47_UO1_HEK_2_1nSAX-ERLICH EnrichmentOrbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS17
MS_47_UO1_HEK_2_2nSAX-ERLICH EnrichmentOrbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS1
MS_51_CM_20210210173056No enrichmentOrbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS8
MS_51_CM2Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS4
MS_51_CM3Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS11
MS_51_HEK_01_EThcDpdHCD_20210204023608Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS16
MS_51_HEK_CM02Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS9
MS_51_HEK_acetoneOrbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS30
MS_51_HEK_acetone_z2inclOrbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS41
MS_51_HEK_acetone2Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS27
MS_51_HEK02_20210214173011Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS4
MS_52_HEK_secretome01Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS15
MS_52_HEK_secretome02Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS6
MS_52_HEK_secretome03Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS14