TG004970

Peptide	R.YPHKPEIN[H5N4S2]STTHPGADLQENFCR.N (PE000550)
Sequence	YPHKPEINSTTHPGADLQENFCR
Glycans(s)	H5N4S2 (N8)
Mod(s)	57.021 (C22)
Molecular Weight	4,933.039
Charge	5+
Protein	F2 (P00734)
Spectrum File	MS_51_ihilic_plasma_1ul
Method	Orbitrap Fusion-Lumos, DDA (EThcD pd HCD), nano LC-MS/MS
Analytical Fraction	iHILIC
Sample	Human Serum

Transitions

Exp. R.T.	Peak R.T.	Norm. R.T.	Prec. m/z	Prec. z	Prod. m/z	Prod. z	Label	%Int
23.355	23.255	15.984	984.016	5+	1,156.994	4+	Y[pH5N4S]	57.4
23.355	23.255	15.984	984.016	5+	1,065.711	4+	Y[pH4N3S]	43.9
23.355	23.255	15.984	984.016	5+	1,084.220	4+	Y[pH5N4]	43.1
23.355	23.255	15.984	984.016	5+	992.937	4+	Y[pH4N3]	41.9
23.355	23.255	15.984	984.016	5+	1,323.580	3+	Y[pH4N3]	37.7
23.355	23.255	15.984	984.016	5+	925.797	5+	Y[pH5N4S]	21.7
23.355	23.255	15.984	984.016	5+	1,269.563	3+	Y[pH3N3]	20.8
23.355	23.255	15.984	984.016	5+	1,201.870	3+	Y[pH3N2]	17.2
23.355	23.255	15.984	984.016	5+	1,147.852	3+	Y[pH2N2]	13.7
23.355	23.255	15.984	984.016	5+	1,043.707	4+	Y[pH4N4]	12.1
23.355	23.255	15.984	984.016	5+	952.424	4+	Y[pH3N3]	8.8
23.355	23.255	15.984	984.016	5+	1,025.198	4+	Y[pH3N3S]	8.3
23.355	23.255	15.984	984.016	5+	972.124	3+	Y[pN]	8.2
23.355	23.255	15.984	984.016	5+	1,116.481	4+	Y[pH4N4S]	7.4
23.355	23.255	15.984	984.016	5+	1,420.612	3+	Y[pH4N3S]	5.9

Spectra

4345;EThcD;23.135;4343;984.219;Byonic:q-value=3.02e-06/GPS:MinICScore=37.700
4347;HCD;23.146;4343;984.219;
4396;EThcD;23.345;4395;984.017;Byonic:q-value=1.44e-06/GPS:MinICScore=26.908
4398;HCD;23.355;4395;984.017;